Synthesis of chitosan-grafted poly-acrylic acid (CTS-g-PAA) hydrogel and its potential application in biosensors for signal enhancing and bioanalysis

Nowadays, hydrogels have been attracted a lot of interest due to their immense potential in different fields such as biomedicine and biotechnology. Biodegradable biocompatible pH-sensitive chitosan-grafted polyacrylic acid (CTS-g-PAA) hydrogel was synthesized by grafting an acrylic monomer onto chitosan at the presence methylene bisacrylamide cross-linking agent ammonium persulphate initiator. FT-IR spectroscopy scanning electron microscopy (SEM) were used analyze properties obtained hydrogel. The is suitable for delivery many hydrophilic drugs or species. Using multi-walled carbon nanotube modified-glassy electrode (CNT-GCE), loading release conditions Nile Blue (NB) electroactive compound evaluated utilizing differential pulse voltammetry (DPV) cyclic (CV). effect various parameters on electrochemical signal NB investigated, optimal efficient performance obtained. electrocatalytic current values show linear dependence concentration range 0.098 – 0.971 ?M while detection limit this platform 12.3 nM. unique proposed with has broad possible applications biosensors enhancement bioanalysis.